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The search for factors in human feeders that support the derivation and propagation of human embryonic stem cells: preliminary studies using transcriptome profiling by serial analysis of gene expression.

Kueh J, Richards M, Ng SW, Chan WK, Bongso A

Department of Obstetrics and Gynecology, National University of Singapore, Kent Ridge, Singapore.

Serial analysis of gene expression (SAGE) was used to obtain the transcriptome profiles of a supportive human fetal skin feeder (Detroit 551) and a nonsupportive human fetal lung feeder (MRC-5) for human embryonic stem cells. A pairwise comparison of the two SAGE profiles showed that fibroblast growth factor-2 (FGF2), a bone morphogenetic protein 4 pathway inhibitor, Gremlin 1, and several extracellular matrix proteins that could potentially aid human embryonic stem cell attachment and growth were highly expressed in Detroit 551 fibroblasts.

Published 8 June 2006 in Fertil Steril, 85(6): 1843-6.
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