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Proteomic profiling of human stem cells derived from umbilical cord blood.

Zenzmaier C, Gesslbauer B, Grobuschek N, Jandrositz A, Preisegger KH, Kungl AJ

Lifecord Inc., Koerblergasse 42, A-8010 Graz, Austria.

CD34+ preparations from five different umbilical cord samples were compared with respect to their proteome profile using 2-D gel electrophoresis. Fifty-two protein spots were found to match in all preparations referring to the high heterogeneity of such samples indicating a not fully developed (or instable) proteome of stem cells. All matching spots were subjected to in-gel digestion and nano-LC-MS/MS sequence analysis, from which 22 proteins were unambiguously identified.

Published 14 February 2005 in Biochem Biophys Res Commun, 328(4): 968-72.
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